BamHI-HF from New England Biolabs

Product Specs
ItemBamHI-HF
CompanyNew England Biolabs
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberR3136S
Concentration20,000 units/ml
Quantity10000 units
ApplicationsDNA Manipulation
Brand/TrademarkNew England Biolabs Inc (NEB)
TypeRestriction Enzyme
Temperature-20 C

Sequence

G⇓GATC C
C CTAG⇑G

Add to Compare List

New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Reshaping the yeast galactose regulon via GPCR signaling cascadeCell Reports MethodsDecember 18, 2023Cong Fan, Jifeng YuanDeoxynucleotide (dNTP) Solution Mix,NEB,Cat#N0447S BsaI-HFv2,NEB,Cat#R3733S T4 DNA Ligase,NEB,Cat#M0202S BamHI,NEB,Cat#R3136S SacI,NEB,Cat#R3156S Shrimp Alkaline Phosphatase (rSAP),NEB,Cat#M0371S Ampicillin Na,Inalco,Cat#1758-9314 S. cerevisiae

(2) Comprehensive identification of onco-exaptation events in bladder cancer cell lines revealed L1PA2-SYT1 as a prognosis-relevant eventiScienceDecember 15, 2023Ziwei Wang, Yidie Ying, Maoyu Wang, Qing Chen, Yi Wang, Xufeng Yu, Wei He, Jing Li, Shuxiong Zeng, Chuanliang Xudigestion of pLVX-IRES-Puro plasmids was performed using restriction enzymes EcoRI and BamHI (R3101S & R3136S, New England BioLabs, Massachusetts, US) according to the manufacturer’s instructions. Predicted L1PA2-SYT1

(3) Genome sequence and characterization of a novel Pseudomonas putida phage, MiCathSci RepDecember 9, 2023James Jaryenneh, Joseph S. Schoeniger, Catherine M. Mageeneyno. N3011S) DNAs were digested with EcoRI-HF (NEB, catalog no. R3101S), BamHI-HF (NEB, catalog no. R3136S), BstXI (NEB, catalog no. R0113S), EcoRV-HF (NEB, catalog no. R3195S), HaeIII (NEB, catalog no. R0108T),

(4) Structural and mechanistic insights into disease-associated endolysosomal exonucleases PLD3 and PLD4bioRxivNovember 24, 2023Meng Yuan, Linghang Peng, Deli Huang, Amanda Gavin, Fangkun Luan, Jenny Tran, Ziqi Feng, Xueyong Zhu, Jeanne Matteson, Ian A. Wilson, et al.pBOBI vector that has a C-terminal FLAG tag after digestion by restriction enzymes BamHI and XhoI (NEB R3136S, R0146S). All these plasmids were prepared with endotoxin-free kit (Takara 740426) and sterile filtered

(5) ZIKV induction of tristetraprolin in endothelial and Sertoli cells post-transcriptionally inhibits IFNβ/λ expression and promotes ZIKV persistencemBioOctober 31, 2023William R. Schutt, Jonas N. Conde, Megan C. Mladinich, Grace E. Himmler, Erich R. Mackowprimers found in Table S1 and inserted between pCW57-MCS1-2A-MCS2 EcoRI (NEB #R3101S) and BamHI (NEB #R3136S) restriction sites. qRT-PCR analysis RNA was purified using RNeasy Mini Kit (Qiagen) according to

(6) Rapid and sensitive one-tube detection of mpox virus using RPA-coupled CRISPR-Cas12 assayCell Reports MethodsOctober 23, 2023Fei Zhao, Yamei Hu, Zhangling Fan, Baoying Huang, Liang Wei, Yu Xie, Yu Huang, Shan Mei, Liming Wang, Lingwa Wang, et al.,Thermo Fisher Scientific,Cat#15070063 DMEM,Hyclone,Cat#SH30243.01 FBS,Hyclone,Cat#SH30396.02 EcoRI-HF,NEB,Cat#R3101S BamHI-HF,NEB,Cat#R3136S GenCRISPR™ Cas12a (Cpf1) Nuclease,GenScript,Cat#Z03502 10xNEBuffer2.1,NEB,Cat# B6002S ,, Critical

(7) Understanding cancer drug resistance with Sleeping Beauty functional genomic screens: Application to MAPK inhibition in cutaneous melanomaiScienceOctober 20, 2023Eliot Y. Zhu, Jacob L. Schillo, Sarina D. Murray, Jesse D. Riordan, Adam J. DupuyR0441 Enzyme: HpaI,NEB,Catalog #: R0105S Enzyme: EcoNI,NEB,Catalog #: R0521S Enzyme: BamH1,NEB,Catalog #: R3136S Enzyme: dsDNA Fragmentase,NEB,Catalog #: M0348 Enzyme: T4 DNA polymerase,NEB,Catalog #: M0203 Enzyme:

(8) Paired yeast one-hybrid assays to detect DNA-binding cooperativity and antagonism across transcription factorsNature CommunicationsOctober 18, 2023Anna Berenson, Ryan Lane, Luis F. Soto-Ugaldi, Mahir Patel, Cosmin Ciausu, Zhaorong Li, Yilin Chen, Sakshi Shah, Clarissa Santoso, Xing Liu, et al.converter plasmid with G418 resistance (Addgene #51680). M3926 was digested with BamHI (New England Biolabs R3136S) and ethanol precipitated. Yα1867 yeast were transformed with digested plasmid as follows. Yeast were

(9) An Animal Model for Chronic Meningeal Inflammation and Inflammatory Demyelination of the Cerebral CortexInternational journal of molecular sciencesSeptember 9, 2023Enz LS, Winkler A, Wrzos C, Dasen B, Nessler S, Stadelmann C, Schaeren-Wiemers N.(D4007, Zymo Irvine, CA, USA). The sequence for lacZ was obtained by restriction digest with BamHI (R3136S, New England Biolabs, Ipswich, MA, USA) of the plasmid LV-lacZ received as a gift from Inder Verma (Addgene

(10) An efficient cloning method to expand vector and restriction site compatibility of Golden Gate AssemblyCell Reports MethodsAugust 22, 2023Masato Sorida, Roberto BonasioGeneRuler 1 kb Plus DNA Ladder,Thermo Scientific,SM1331 BsaI-HFv2,NEB,R3733S BbsI-HF,NEB,R3539S AgeI-HF,NEB,R3552S BamHI-HF,NEB,R3136S EcoRI-HF,NEB,R3101L HindIII-HF,NEB,R3104S NcoI-HF,NEB,R3193S NotI-HF,NEB,R3189S SalI-HF,NEB,R3138S SpeI-HF,NEB,R3133S XbaI,NEB,R0145S XhoI,NEB,R0146S XmaI,NEB,R0180S KpnI-HF,NEB,R3142S NdeI,NEB,R0111S Smal,NEB,R0141S BbsI-HF,NEB,R3539M T4 DNA Ligase

(11) High-sensitive nascent transcript sequencing reveals BRD4-specific control of widespread enhancer and target gene transcriptionNat CommunAugust 17, 2023Annkatrin Bressin, Olga Jasnovidova, Mirjam Arnold, Elisabeth Altendorfer, Filip Trajkovski, Thomas A. Kratz, Joanna E. Handzlik, Denes Hnisz, Andreas MayerpGL3-promoter vector by either conventional restriction enzyme cloning using BamHI-HF or SalI-HF (NEB, Cat.# R3136S and R3138S) or by Gibson Assembly (NEB, Cat.# E2611S). The primer sequences used for amplification are

(12) Fucosylated glycoproteins and fucosylated glycolipids play opposing roles in cholera intoxicationbioRxivAugust 3, 2023Atossa C. Ghorashi, Andrew Boucher, Stephanie A. Archer-Hartmann, Nathan B. Murray, Rohit Sai Reddy Konada, Xunzhi Zhang, Chao Xing, Parastoo Azadi, Ulf Yrlid, Jennifer J. Kohlerencoding lentiviral vector (Addgene, plasmid no. 52962) cut with BamHI-HF and AgeFI (NEB, catalog no. R3136S and R0652S, respectively) according to manufacturer’s guidelines. Plasmids were transformed into DH5α

(13) Long-range DNA end resection supports homologous recombination by checkpoint activation rather than extensive homology generationeLifeJune 30, 2023Michael T Kimble, Matthew J Johnson, Mattie R Nester, Lorraine S Symingtonsection) other,AatII,New England BioLabs,Cat# R0117L,Restriction enzyme other,BamHI-HF,New England BioLabs,Cat# R3136S,Restriction enzyme other,BglII,New England BioLabs,Cat# R0144S,Restriction enzyme other,EcoRV-HF,New England BioLabs

(14) Fusion crystallization reveals the behavior of both the 1TEL crystallization chaperone and the TNK1 UBA domainbioRxivJune 18, 2023Supeshala Nawarathnage, Yi Jie Tseng, Sara Soleimani, Tobin Smith, Maria J Pedroza Pedroza Romo, Wisdom Oshireku Abiodun, Christina M. Egbert, Deshan Madhusanka, Derick Bunn, Bridger Woods, et al.cells,NEB,Cat# C2527H ,, ,, ,, "Chemicals, peptides, and recombinant proteins",, BamHI-HF,NEB,Cat# R3136S CutSmart Buffer,NEB,Cat# B7204S Xhol,NEB,Cat# R0146S NdeI,NEB,Cat# R0111S Gibson master mix,D.G.

(15) eIF3 mRNA selectivity profiling reveals eIF3k as a cancer-relevant regulator of ribosome contentThe EMBO journalJune 15, 2023Duan H, Zhang S, Zarai Y, Öllinger R, Wu Y, Sun L, Hu C, He Y, Tian G, Rad R, et al.,New England Biolabs,Cat # R0145S XhoI,New England Biolabs,Cat # R0146S BamHI,New England Biolabs,Cat # R3136S T4 Polynucleotide Kinase,New England Biolabs,Cat # M0201S T4 DNA Ligase,New England Biolabs,Cat #

(16) Mutational screens highlight glycosylation as a modulator of colony-stimulating factor 3 receptor (CSF3R) activityJournal of Biological ChemistryJune 1, 2023Michael J. Hollander, Stacy A. Malaker, Nicholas M. Riley, Idalia Perez, Nayla M. Abney, Melissa A. Gray, Julia E. Maxson, Jennifer R. Cochran, Carolyn R. Bertozzirestriction sites. After sequence verification, plasmids were digested with BamHI-HF (New England Biolabs R3136S)/MfeI-HF (New England Biolabs R3589S) (gp130), MfeI-HF/AgeI-HF (New England Biolabs R3552S) (LIFR),

(17) Structural conservation of Lassa virus glycoproteins and recognition by neutralizing antibodiesCell ReportsMay 30, 2023Hailee R. Perrett, Philip J.M. Brouwer, Jonathan Hurtado, Maddy L. Newby, Lin Liu, Helena Müller-Kräuter, Sarah Müller Aguirre, Judith A. Burger, Joey H. Bouhuijs, Grace Gibson, et al.,Molecular Devices,N/A ,, Other,Other,Other ,, PstI-HF,New England Biolabs,Cat# R3140S BamHI-HF,New England Biolabs,Cat# R3136S NotI-HF,New England Biolabs,Cat# R3189 Q5 Site-directed mutagenesis kit,New England Biolabs,Cat# E0554S T4 DNA ligase

(18) AKT activation because of PTEN loss upregulates xCT via GSK3β/NRF2, leading to inhibition of ferroptosis in PTEN-mutant tumor cellsCell ReportsMay 20, 2023Kaitlyn M. Cahuzac, Abigail Lubin, Kaitlyn Bosch, Nicole Stokes, Sarah Mense Shoenfeld, Royce Zhou, Haddy Lemon, John Asara, Ramon E. ParsonsDoxycycline hyclate,Sigma,D9891-25G Sulfasalazine,Sigma,S0883 RSL3,Sigma,SML2234 Erastin,Sigma,E7781 Bafilomycin,Sigma,SML1661 GDC0941,Sigma,"957,054–30-7" L-glutamine,Fisher,MT25005CI polybrene,Millipore Sigma,TR-1003-G Blasticidin HCl,ThermoFisher,A1113903 BamHI-HF,New England BioLabs,R3136S EcoRI-HF,New England BioLabs,R3101S MluI-HF,New England BioLabs,R3198S BsmBI,New England BioLabs,R0739S NotI-HF,New England BioLabs

(19) Novel Antibody-independent Method to Measure Complement Deposition on BacteriaBIO-PROTOCOLMay 5, 2023Toksa Wonfor, Shuxian Li, Maisem Laabei1704158)V-bottomed 96-well plate (Thermo Fisher, Nunc, catalog number: 249570)BamHI-HF (NEB, catalog number: R3136S)BL21 DE3 competent cells (NEB, catalog number: C2527I). Alternatively, make your own competent cells

(20) Probing Oxidant Effects on Superoxide Dismutase 1 Oligomeric States in Live Cells Using Single-Molecule Fluorescence AnisotropyChemical & Biomedical ImagingApril 24, 2023Huanhuan Chen, Tai-Yen Chenthe mEos4b-N1 plasmid was then replaced by PCR-amplified mEos4b-FLAG between cutting sites BamHI (NEB R3136S) and Notl (NEB R3189S) to generate the monomeric mEos4b construct. To generate the dmE construct, we

Write a Review

(1)

4/16/2018Diagnostic Restriction Mapping Of Plasmid Constructs Triveni MahendrappaI have constructed a plasmid using pAYC184 as backbone with 16 kb operon making it to 20 kb size. I wanted to quickly check the constructed plasmid with restriction with BamHI and SalI double digest. ... Read Review

BamHI-HF

BamHI-HF from New England Biolabs

Description

Sequence

New England Biolabs

Citations (128)

Reviews

BamHI-HF from New England Biolabs

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map